AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |
Back to Blog
Cs 1.6 original nemodificat12/31/2022 To asses if these proteins were specifically related to n-hexadecane metabolism, their expression was investigated, comparing H proteome with that of M145-AH incubated in BH with glucose as sole carbon source (G condition). Proteome analysis revealed significant changes only at 48 h, showing 48 differentially abundant proteins identified by mass spectrometry procedures. To unravel proteins associated with growth on n-alkanes, proteome of M145-AH after 6, 24, and 48 h of incubation in the Bushnell-Haas (BH) mineral medium containing n-hexadecane as sole carbon source (H condition) and in BH without any carbon source (0 condition) were compared using 2D-differential gel electrophoresis. The resulting engineered strain, M145-AH, can grow on n-hexadecane as sole carbon source. SoCg, was expressed in the non-alkane-degrading actinomycete Streptomyces coelicolor M145. The alkB gene, encoding an alkane monooxygenase in the actinomycete Gordonia sp.
0 Comments
Read More
Leave a Reply. |